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_data/sidebars/user_guide.yml

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subitems:
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- title: Creating an ISA-JSON compliant study
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url: /help/user-guide/studies
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- title: Export experiments as ISA-JSON
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url: /help/user-guide/exporting-experiments-as-isajson
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- title: Assays
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subitems:
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- title: Creating an ISA-JSON compliant assay
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url: /help/user-guide/assays
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- title: Export experiments as ISA-JSON
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url: /help/user-guide/exporting-experiments-as-isajson
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- title: Experiment Sample Templates
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subitems:
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- title: Creating Experiment Sample Templates

help/user-guide/adding-assets.md

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* [Tags](general-attributes#tags)
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* [Attributions](general-attributes#attributions)
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* [Creators](general-attributes#creators)
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* [SOPs](general-attributes#sops)
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* [SOPs](sops)
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* [Data files](general-attributes#data-files)
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* [Publications](general-attributes#publications)
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* [Publications](publications)
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* [Experimental assays and Modelling analysis](general-attributes#experimental-assays-and-modelling-analysis)
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* [Events](general-attributes#events)

help/user-guide/assays.md

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---
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title: Asssays
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title: Assays
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---
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The [ISA metadata framework](https://isa-specs.readthedocs.io/en/latest/isamodel.html) requires the description (metadata) of different types of samples, namely Study Sources, Study Samples and Assay Samples. This description is based on customisable Experiment Sample Templates and includes the linking of applied Protocols. Follow the link for an overview about [ISA-JSON compliant experiments](isa-json-compliant-experiment.html).
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* Select an Assay Stream, then click on "Design Assay" button at the top right corner of the page.
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* Fill out the provided form as explained below.
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### 2.1 Link the sampling Protocol
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Select Protocols already registered in the platform that describe the used method or procedure (SOP) applied to the Assay. See how to [create an SOP](adding-assets) in SEEK.
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### 2.1 Link the sampling Protocol
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Select Protocols already registered in the platform that describe the used method or procedure (SOP) applied to the Assay. See how to [create an SOP](sops) in SEEK.
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### 2.2 Design a Samples table for Assay
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* assay - data file: if the output of the assays are digital data files
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* organism
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![create isaassay 2](/images/user-guide/isajson-compliance/create_isaassay_2.png){:.screenshot}
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![create isaassay 2]({{ "/images/user-guide/isajson-compliance/create_isaassay_2.png" | relative_url }}){:.screenshot}
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* Choose a template from the resulting dropdown menu.
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* Select "Apply".
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* If you want to add new attributes of your choice to your Samples table, select “Add new attribute” button.
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* Fill out the mandatory and optional fields. Note that for ISA-JSON compliant Experiments, the ISA Tag is a mandatory field.
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* For ISA tag, select
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* For ISA tag, select
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* in case of ISA Level "assay - material": "other_material_characteristic" or "parameter_value";
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* in case of ISA Level "assay - data file": "data_file_comment" or "parameter_value";
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Note that selecting any other options would generate an error since other options are already selected in the starting template.
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![create isaassay 3](/images/user-guide/isajson-compliance/create_isaassay_3.png){:.screenshot}
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![create isaassay 3]({{ "/images/user-guide/isajson-compliance/create_isaassay_3.png" | relative_url }}){:.screenshot}
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### 2.3 Visualise ISA Assay
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Upon creation, the newly designed ISA Assay will appear in the tree view on the left sidebar, in Experiment View. Follow the link to know more about [Experiment View](viewing-project-in-single-page).
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## 3. Adding samples to ISA Assay
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Follow the link to know how to [create samples in ISA-JSON compliant experiments](create-sample-isajson-compliant), including [Assay Samples](create-sample-isajson-compliant#create-assay-samples).
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If the assay is the last one in the assay stream, it can be removed without affecting the structure of the stream. If it is a midway assay (i.e., not the last assay in the assay stream), the system will automatically update the sample linkage. The input sample of the following assay will always link to the output sample of the preceding assay without requiring manual intervention. To remove, select the assay and click “Actions -> Delete ISA Assay” in the top right corner.
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### 4.2 Adding an Assay
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Adding a new assay is straightforward when it follows the last assay in the stream. This is permitted even if samples are associated with the assay. Select the last assay in the assay stream, and click the “Design the next Assay” button at the top right.
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For adding a midway assay, the next assay in the stream cannot have samples associated with it. Sample linkage is updated automatically, and no manual re-establishment is needed. To add a midway assay, select the preceding assay where you want to add an additional assay, and select “Insert a new Assay”. The new assay will be added after the selected assay.
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Adding a new assay is straightforward when it follows the last assay in the stream. This is permitted even if samples are associated with the assay. Select the last assay in the assay stream, and click the “Design the next Assay” button at the top right.
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For adding a midway assay, the next assay in the stream cannot have samples associated with it. Sample linkage is updated automatically, and no manual re-establishment is needed. To add a midway assay, select the preceding assay where you want to add an additional assay, and select “Insert a new Assay”. The new assay will be added after the selected assay.
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### 4.3 Editing an Assay
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Editing an assay follows similar rules to deletion. If an assay has associated samples, certain modifications to the metadata attributes may be restricted. However, it possible to add metadata attributes, or edit the assay’s title, description, etc.
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Editing an assay follows similar rules to deletion. If an assay has associated samples, certain modifications to the metadata attributes may be restricted. However, it possible to add metadata attributes, or edit the assay’s title, description, etc.

help/user-guide/create-sample-isajson-compliant.md

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* In ISA Assay, Experiment overview shows Study Samples and Assay samples, up until that experimental step.
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## Browsing samples by Experiment Sample templates
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[Browsing samples by experiment sample templates](browsing#browsing-samples-by-experiment-sample-templates)
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[Browsing samples by experiment sample templates](browsing#browse-samples-by-experiment-sample-template)

help/user-guide/investigations.md

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In the context of an ISA-JSON compliant experiment, we use the terms ISA Investigation, ISA Study, and ISA Assay when referring to Investigation, Study, and Assay, respectively.
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## 1. Creating an ISA Investigation
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Select
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Select
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* Create Investigation from header menu bar.
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* Alternatively, in Experiment View, select the Design Investigation button at the top right corner
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* Alternatively, in Experiment View, select the Design Investigation button at the top right corner
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Fill out the provided form, check the option for "Make Investigation compliant to ISA-JSON schemas?" and then click the 'Create' button.
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![select isajson compliance](/images/user-guide/isajson-compliance/select_isajson_compliance.png){:.screenshot}
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![select isajson compliance]({{ "/images/user-guide/isajson-compliance/select_isajson_compliance.png" | relative_url }}){:.screenshot}
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help/user-guide/isa-json-compliant-experiment.md

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![select isajson compliance]({{ "/images/user-guide/isajson-compliance/select_isajson_compliance.png" | relative_url }}){:.screenshot}
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For a step-by-step description on how to set up ISA-JSON compliant Investigation, see [Creating an ISA-JSON compliant Investigation](investigations).
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## ISA Study
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ISA Study can only be associated to an ISA Investigation. It is not possible to associate an ISA Study to an Investigation which is not ISA-JSON compliant.
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3. *Sample(s)* - the physical material which results from the sampling protocol (e.g. leaves, biopsies, aliquotes).
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For a step-by-step description on how to set up ISA-JSON compliant Study, see [Creating an ISA-JSON compliant Study](studies).
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## Assay Stream
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An Assay Stream constitutes a structured sequence of sequential assays, interconnected through the flow of samples. Within an Assay Stream, the sample output of one assay serves as the input for the subsequent one. Each Assay Stream aligns with a single Assay in the ISA metadata framework. It is typically associated with one specific technology or technique, such as Metabolomics or Sequencing.
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* physical materials (e.g. nucleic acids extracts, RNA libraries) generated from an experimental step;
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For a step-by-step description on how to set up ISA-JSON compliant Assay, see [Creating an ISA-JSON compliant Assay](assays).
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## Experiment Sample Templates
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See [Working with Samples in ISA-JSON compliant Experiments](create-sample-isajson-compliant) for more information.
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## Designing ISA-JSON compliant Experiments
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## ISA-JSON export
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[Export Experiments as ISA-JSON](exporting-experiments-as-isajson)
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help/user-guide/publications.md

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## Publications
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If your asset is directly related to a publication you can link the two together in SEEK. You can select publications within your project form the drop-down menu. If the publication is in another project you need to check the box that says associate publications from other projects.
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![add publication 1](/images/user-guide/add_publication_1.png){:.screenshot}
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![add publication 1]({{ "/images/user-guide/add_publication_1.png" | relative_url }}){:.screenshot}
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When a publication is added a preview will be shown in the bottom right hand corner of SEEK. It can be removed easily if needed.
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![add publication 2](/images/user-guide/add_publication_2.png){:.screenshot}
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![add publication 2]({{ "/images/user-guide/add_publication_2.png" | relative_url }}){:.screenshot}

help/user-guide/studies.md

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## 21. Creating an ISA Study
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## 1. Creating an ISA Study
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## 1.1 Design a Sources table for Study Sources
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### 1.1 Design a Sources table for Study Sources
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The Study Sources table is a Sample Type associated with the Study and can only be accessed through the Study interface. The Study Sources table can only be created starting from an existing Experiment Sample Templates.
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### 1.1.1 Choose one Experiment Sample Template
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#### 1.1.1 Choose one Experiment Sample Template
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![create isastudy source 1](/images/user-guide/isajson-compliance/create_isastudy_source_1.png){:.screenshot}
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![create isastudy source 1]({{ "/images/user-guide/isajson-compliance/create_isastudy_source_1.png" | relative_url }}){:.screenshot}
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* Filter existing Experiment Sample Templates based on:
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![create isastudy source 2]({{ "/images/user-guide/isajson-compliance/create_isastudy_source_2.png" | relative_url }}){:.screenshot}
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#### 1.1.2 Customise the Study Sources table
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![create isastudy source 3](/images/user-guide/isajson-compliance/create_isastudy_source_3.png){:.screenshot}
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![create isastudy source 3]({{ "/images/user-guide/isajson-compliance/create_isastudy_source_3.png" | relative_url }}){:.screenshot}
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## 1.2 Link the sampling Protocol
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Select Protocols already registered in the platform that describe the used method or procedure (SOP) used to collect Samples from Sources in your Study (Samples collection protocol). See how to [create an SOP](adding-assets) in SEEK.
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### 1.2 Link the sampling Protocol
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Select Protocols already registered in the platform that describe the used method or procedure (SOP) used to collect Samples from Sources in your Study (Samples collection protocol). See how to [create an SOP](sops) in SEEK.
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## 1.3 Design a Samples table for Study Samples
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### 1.3 Design a Samples table for Study Samples
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## 1.4 Visualise ISA Study
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### 1.4 Visualise ISA Study
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Upon creation, the newly designed ISA Study will appear in the tree view on the left sidebar, in Experiment View. Follow the link to know more about [Experiment View](viewing-project-in-single-page).
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## 2. Adding Sources to ISA Study
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Follow the link to know how to [create samples in ISA-JSON compliant experiments](create-sample-isajson-compliant), including Study Sources.
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## 3. Adding Samples to ISA Study
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Follow the link to know how to [create samples in ISA-JSON compliant experiments](create-sample-isajson-compliant), including [Study Samples](create-sample-isajson-compliant#create-study-samples).
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Follow the link to know how to [create samples in ISA-JSON compliant experiments](create-sample-isajson-compliant), including [Study Samples](create-sample-isajson-compliant#create-study-samples).

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